Since a Y chromosome specifies testes, and since the presence or absence of the testes' secretions specifies the remaining male or female structures, it might seem as if there's no way that a developing human could end up with ambiguous sexual anatomy. Instead, you might think that a Y chromosome should guarantee 100 percent male organs, and that lack of a Y chromosome should guarantee 100 percent female organs.

In fact, a long series of biochemical steps is required to produce all those other structures besides ovaries or testes. Each step involves the synthesis of one molecular ingredient, termed an enzyme, specified by one gene. Any enzyme can be defective or absent if its underlying gene is altered by a mutation. Thus, an enzyme defect may result in a male pseudohermaphrodite, defined as someone possessing some female structures as well as testes. In a male pseudoher-maphrodite with an enzyme defect, there is normal development of the male structures dependent on enzymes that act at the steps of the metabolic pathway before the defective enzyme. However, male structures dependent on the defective enzyme itself or on subsequent biochemical steps fail to develop and are replaced either by their female equivalent or by nothing at all. For example, one type of pseudohermaphrodite looks like a normal woman. Indeed, “she” conforms to the male ideal of female pulchritude even more closely than does the average real woman, because “her” breasts are well developed and “her” legs are long and graceful. Hence cases have turned up repeatedly of beautiful women fashion models not realizing that they are actually men with a single mutant gene until genetically tested as adults.

Since this type of pseudohermaphrodite looks like a normal girl baby at birth and undergoes externally normal development and puberty, the problem isn't even likely to be recognized until the adolescent “girl” consults a doctor over failure to begin menstruating. At that point, the doctor discovers a simple reason for that failure: the patient has no uterus, fallopian tubes, or upper vagina. Instead, the vagina ends blindly after two inches. Further examination reveals testes that secrete normal testosterone, are programmed by a normal Y chromosome, and are abnormal only for being buried in the groin or labia. In other words, the beautiful model is an otherwise normal male who happens to have a genetically determined biochemical block in his ability to respond to testosterone.

That block turns out to be in the cell receptor that would normally bind testosterone and dihydrotestos-terone, thereby enabling those androgens to trigger the further developmental steps of the normal male. Since the Y chromosome is normal, the testes themselves form normally and produce normal Mullerian inhibiting hormone, which acts as in any man to forestall development of the uterus and fallopian tubes. However, development of the usual male machinery to respond to testosterone is interrupted. Hence development of the remaining bipotential embryonic sex organs follows the female channel by default: female rather than male external genitalia, and atrophy of the Wolffian ducts and hence of potential male internal genitalia. In fact, since the testes and adrenal glands secrete small amounts of estrogen that would normally be overridden by androgen receptors, the complete lack of those receptors in functional form (they are present in small numbers in normal women) makes the male pseudohermaphrodite appear externally superfeminine.

Thus, the overall genetic difference between men and women is modest, despite the big consequences of that modest difference. A small number of genes on chromosome 23, acting in concert with genes on other chromosomes, ultimately determine all differences between men and women. The differences, of course, include not just those in the reproductive organs themselves but also all other postadolescent sex-linked differences, such as the differences in beards, body hair, pitch of voice, and breast development.

The actual effects of testosterone and its chemical derivatives vary with age, organ, and species. Animal species differ greatly in how the sexes differ, and not only in mammary gland development. Even among higher anthropoids— humans and our closest relatives, the apes-there are familiar differences in sexual distinctiveness. We know from zoos and photos that adult male and female gorillas differ obviously at a long distance by the male's much greater size (his weight is double the female's), different shape of head, and silver-haired back. Men also differ, though much less obviously, from women in being slightly heavier (by 20 percent on the average), more muscular, and bearded. Even the degree of that difference varies among human populations: for example, the difference is less marked among Southeast Asians and Native Americans, since men of those populations have on the average much less body hair and beard development than in Europe and Southwest Asia. But males and females of some gibbon species look so similar that you couldn't distinguish them unless they permitted you to examine their genitals.

In particular, both sexes of placental mammals have mammary glands. While the glands are less well developed and nonfunctional in males of most mammal species, that degree of male underdevelopment varies among species. At the one extreme, in male mice and rats, the mammary tissue never forms ducts or a nipple and remains invisible from the outside. At the opposite extreme, in dogs and primates (including humans) the gland does form ducts and a nipple in both males and females and scarcely differs between the sexes before puberty.

During adolescence the visible differences between the mammalian sexes increase under the influence of a mix of hormones from the gonads, adrenal glands, and pituitary gland. Hormones released in pregnant and lactating females produce a further mammary growth spurt and start milk production, which is then reflexly stimulated by nursing. In humans, milk production is especially under the control of the hormone prolactin, while the responsible hormones in cows includes somatotropin, alias “growth hormone” (the hormone behind the current debate over proposed hormonal stimulation of milk cows).

It should be emphasized that male/female differences in hormones aren't absolute but a matter of degree: one sex may have higher concentrations and more receptors for a particular hormone. In particular, becoming pregnant is not the only way to acquire the hormones necessary for breast growth and milk production. For instance, normally circulating hormones stimulate a milk production, termed witch's milk, in newborns of several mammal species. Direct injection of the hormones estrogen or progesterone (normally released during pregnancy) triggers breast growth and milk production in virgin female cows and goats-and also in steers, male goats, and male guinea pigs. The hor-monally treated virgin cows produced on the average as much milk as their half-sisters that were nursing calves to which they had given birth. Granted, hormonally treated steers produced much less milk than virgin cows; you shouldn't count on steer's milk in the supermarkets by next Christmas. But that's not surprising since the steers have previously limited their options: they haven't developed an udder to accommodate all the mammary gland tissue that hormonally treated virgin cows can accommodate.

There are numerous conditions under which injected or topically applied hormones have produced inappropriate breast development and milk secretion in humans, both in men and in nonpregnant or non-nursing women. Men and women cancer patients being treated with estrogen proceeded to secrete milk when injected with prolactin; among such patients was a sixty-four-year-old man who continued to produce milk for seven years after hormonal treatment was discontinued. (This observation was made in the 1940s, long before the regulation of medical research by human subjects protection committees, which now forbid such experiments). Inappropriate lactation has been observed in people taking tranquilizers that influence the hypothalamus (which controls the pituitary gland, the source of prolactin); it also has been observed in people recovering from surgery that stimulated nerves related to the suckling reflex, as well as in some women on prolonged courses of estrogen and progesterone birth-control pills. My favorite case is the chauvinist husband who kept complaining about his wife's “miserable little breasts,” until he was shocked to find his own breasts growing. It turned out that his wife had been lavishly applying estrogen cream to her breasts to stimulate the growth craved by her husband, and the cream had been rubbing off on him.